Author CINN

The most innovative designers consciously reject the standard option box and cultivate an appetite for thinking wrong.

07

Dec 2018

Quantification of Global DNA Methylation Levels by Mass Spectrometry

Posted by / in Publicaciones 2018, Publicaciones Destacadas, Publicaciones Nanomedicina, Publications Nanomedicine 2018, Últimas Publicaciones CINN / No comments yet

Global DNA methylation was classically considered the relative percentage of 5-methylcysine (5mC) with respect to total cytosine (C). Early approaches were based on the use of high-performance separation technologies and UV detection. However, the recent development of protocols using mass spectrometry for the detection has increased sensibility and permitted the precise identification of peak compounds based on their molecular masses. This allows work to be conducted with much less genomic DNA starting material and also to quantify 5-hydroxymethyl-cytosine (5hmC), a recently identified form of methylated cytosine that could play an important role in active DNA demethylation. Here, we describe the protocol that we currently use in our laboratory to analyze 5mC and 5hmC by mass spectrometry. The protocol, which is based on the method originally developed by Le and colleagues using Ultra Performance Liquid Chromatography (UPLC) and mass spectrometry (triple Quadrupole (QqQ)) detection, allows for the rapid and accurate quantification of relative global 5mC and 5hmC levels starting from just 1 μg of genomic DNA, which allows for the rapid and accurate quantification of relative global 5mC and 5hmC levels.

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07

Dec 2018

Synthesis, crystal structure, characterizations and magnetic study of a novel two-dimensional iron fluoride

Posted by / in Publicaciones 2018, Publicaciones Materiales Multifuncionales 2018, Publicaciones: Nanocomposites Multifuncionales, Últimas Publicaciones CINN / No comments yet

A new hybrid compound formulated as [Fe3F8(H2O)2](Am2TAZ)2 (Am2TAZ= 3,5-diamino-1,2,4-triazole) was prepared under hydrothermal conditions. The crystal structure was solved by single-crystal X-ray diffraction and the bulk was characterized by thermal analyses (TG-MS), vibrational spectroscopy (FTIR, Raman), Ultraviolet-visible spectroscopy (UV–Vis), and scanning electron microscopy (SEM-EDX). It crystallizes in the triclinic system space group P with unit cell parameters a= 7.100(2) Å, b= 7.658(2) Å, c= 8.321(2) Å, α = 107.330(20)°, β = 111.842(18)°, γ = 93.049(17)°, Z = 1 and V= 394.01(17) Å3. The studied X-ray crystal structure shows the two oxidation states for iron atoms (Fe2+, Fe3+) and generates a 2D inorganic network, built up of inorganic layers constructed from infinite inorganic chains running along a axis. In fact, these chains are connected via (Fe3+(3)F6) octahedral. OW-H…F and N-H…F hydrogen bonds, making up the whole 3D network, are strongly linked in the layers. Magnetization measurements were performed, exhibiting the paramagnetic feature of the studied compound above 150 K.

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07

Dec 2018

Synthesis, crystal structure, thermal analysis, dielectric properties and electrical conduction mechanisms of the new mixed solid solution of thallium rubidium sulfate selenate tellurate

Posted by / in Publicaciones 2018, Publicaciones Materiales Multifuncionales 2018, Publicaciones: Nanocomposites Multifuncionales, Últimas Publicaciones CINN / No comments yet

The new mixed compound Tl1.90Rb0.10(SO4)0.92(SeO4)0.08Te(OH)6 (TlRbSSeTe) has been synthesized in order to determine the temperature transitions and to specify the electrical properties as well as the conduction mechanism. It is obtained by slow evaporation at room temperature and prove to crystallize in the monoclinic system with P21/c space group using X-ray diffractometer data. The basic property of these atomic arrangements is the coexistence of three different anions (, and groups) in the unit cell, related by O−H…O hydrogen bonds building up the crystal. Thermal analysis (DSC, DTA and TG) of the title compound confirms the presence of the phase transitions and the temperature of the decomposition of the studied salt. In order to characterize the phase transitions, Raman spectra have been recorded at various temperatures in the wavenumber range from 50–1200 cm−1. Detailed dielectric and electrical properties of the material have been analyzed as a function of frequency (100 Hz–10 MHz) and temperature (383–518 K). Besides, the analysis of Nyquist plots were well fitted to an equivalent circuit consisting of a series of combination of grains and grain boundary elements. The variation of the DC and AC conductivity confirms all the transitions of our sample. Furthermore, The frequency dependence of alternative current (AC) conductivity is interpreted in terms of Jonscher’s law (developed). The alternative current (AC) electrical conduction in TlRbSSeTe material is accounted for in terms of two processes that can be assigned to a hopping transport mechanism. These processes are the correlated barrier hopping (CBH) model in some regions, as well as the non-overlapping small polaron tunneling (NSPT) model in the other regions. The conduction mechanism for each phase is determined with the help of Elliot’s theory.

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