Publications: Nanomedicine

Publications of the research group on nanomedicine

15

Sep 2017

Osseous differentiation on freeze casted 10CeTZP-Al2O3 structures

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Three-dimensional structures with directionally oriented pore networks were fabricated from a 10 mol% ceria-stabilized zirconia and alumina composite (10CeTZP-Al2O3) via freeze casting. Ceramic suspensions of different concentrations (30, 40 and 50 wt% solids) were frozen at various rates (2, 5 and 10 °C/min) to obtain lamellar structures with aligned tubular pores of different characteristics: porosity (75–84%), pore dimensions (small diameter of the elliptical pores: 10–23 μm; large diameter of the elliptical pores: ∼200 ± 70 μm), lamella thickness (2.7–4 μm) and compression strength (1–12 MPa). In vitro assays confirmed the non-cytotoxic nature of the samples. Furthermore, specific osseous differentiation genes were quantified after incubating osteoblasts on different cross sections of the samples during 7 days in supplemented culture medium; results demonstrated that the freeze casted structures induce up to nine times more osseous gene expression than tissue culture polystyrene (TCPS), an advanced surface used for optimized in vitro cell growth.

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24

Aug 2017

The role of 5-hydroxymethylcytosine in development, aging and age-related diseases

Posted by / in Featured Publications, Latest CINN Publications, Publications 2017, Publications Nanomedicine, Publications Nanomedicine 2017 / No comments yet

DNA methylation at the fifth position of cytosines (5mC) represents a major epigenetic modification in mammals. The recent discovery of 5-hydroxymethylcytosine (5hmC), resulting from 5mC oxidation, is redefining our view of the epigenome, as multiple studies indicate that 5hmC is not simply an intermediate of DNA demethylation, but a genuine epigenetic mark that may play an important functional role in gene regulation. Currently, the availability of platforms that discriminates between the presence of 5mC and 5hmC at single-base resolution is starting to shed light on the functions of 5hmC. In this review, we provide an overview of the genomic distribution of 5hmC, and examine recent findings on the role of this mark and the potential consequences of its misregulation during three fundamental biological processes: cell differentiation, cancer and aging.

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17

Jul 2017

Interindividual epigenetic variability: Sound or noise?

Posted by / in Latest CINN Publications, Publications 2017, Publications Nanomedicine, Publications Nanomedicine 2017 / No comments yet

Interindividual variability is an inherent characteristic of biological systems. Whereas the underlying molecular sources of interindividual variability remain poorly understood, recent work by Ecker et al. (Ecker S, Chen L, Pancaldi V, Bagger FO, et al. 2017. Genome Biol 18: 18.) sheds light on the characterization of this phenomenon in a complex biological scenario. By combining data from the BLUEPRINT Epigenome Project with a novel analytical approach, these authors were able to measure the degree of transcriptional and epigenetic variability across a wide panel of samples and types of immune cell. Interestingly, neutrophils displayed increased variability compared to monocytes and T cells, which may be related to the crucial role of the former as an initial mediator of immune responses. Here we review recent literature in this area, and discuss some important issues raised by these innovative analyses. Furthermore, we summarize other potential sources of epigenetic variability, such as epigenetic drift and the epigenetic clock, as well as the current ongoing direction of the field.

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